Anticorps Polyclonal de lapin anti-TRAPPC3

• Phare
• Validé par KD/KO

TRAPPC3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15555-1-AP

Synonymes

BET3, BET3 homolog, TRAPPC3

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Galerie de données de validation

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  WB analysis of HEK-293 using 15555-1-AP

  WB result of TRAPPC3 antibody (15555-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC3 transfected HEK-293 cells.

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  WB analysis of mouse liver using 15555-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 15555-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15555-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15555-1-AP (TRAPPC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15555-1-AP

  IP Result of anti-TRAPPC3 (IP:15555-1-AP, 3ug; Detection:15555-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15555-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15555-1-AP (TRAPPC3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 15555-1-AP

  Immunofluorescent analysis of Hela cells, using TRAPPC3 antibody 15555-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules PC-3, tissu d'intestin grêle de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

15555-1-AP cible TRAPPC3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRAPPC3 Protéine recombinante Ag7924
• Nom complet: trafficking protein particle complex 3
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 20-22 kDa
• Numéro d’acquisition GenBank: BC007662
• Symbole du gène: TRAPPC3
• Identification du gène (NCBI): 27095
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPPC3 (trafficking protein particle complex 3, also known as Bet3) is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. There are three TRAPP complexes identified in yeast with distinct roles: TRAPPI in ER-Golgi traffic, TRAPPII in intra-Golgi and endosome-Golgi traffic, and TRAPPIII in autophagy. Recently it has been proposed that at least two complexes exist in mammals. TRAPPC3 is the most conserved subunit of TRAPP and has been used to precipitate the intact tethering complex both from yeast and from human cells. It has also been reported that TRAPPC3 is required for Rabin8 centrosome trafficking and ciliogenesis. Expressed ubiquitously, TRAPPC3 protein is present in both membrane-bound and cytosolic forms. This antibody recognizes the endogenous 20-22 kDa TRAPPC3 in multiple cell lines. (15728249, 21273506, 23394947)

Publications

Species	Application	Title
human	WB	Cell; A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility.; Authors - Bassik Michael C MC
human	WB	Proc Natl Acad Sci U S A; Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.; Authors - Westlake Christopher J CJ
mouse	WB	Int J Biol Sci; Defective neurite elongation and branching in Nibp/Trappc9 deficient zebrafish and mice; Authors - Min Hu
human	WB	J Biol Chem; The C7orf43/TRAPPC14 component links the TRAPPII complex to RABIN8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.; Authors - Adrian Cuenca
human	WB	Signal Transduct Target Ther; RING finger 138 deregulation distorts NF-кB signaling and facilities colitis switch to aggressive malignancy.; Authors - Yalan Lu