Anticorps Recombinant de lapin anti-TRAPPC4

• Phare
• Validé par KD/KO

TRAPPC4 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

241158C11

N° de cat : 84119-3-RR

Synonymes

241158C11, CGI 104, CGI-104, HSPC172, PTD009

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Galerie de données de validation

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  WB analysis of MCF-7 using 84119-3-RR

  WB result of TRAPPC4 antibody (84119-3-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

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  WB analysis using 84119-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84119-3-RR (TRAPPC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 84119-3-RR

  IP result of anti-TRAPPC4 (IP:84119-3-RR, 4ug; Detection:84119-3-RR 1:3000) with HL-60 cells lysate 2240 ug.

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  IHC staining of human colon cancer using 84119-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84119-3-RR (TRAPPC4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84119-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84119-3-RR (TRAPPC4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84119-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRAPPC4 antibody (84119-3-RR, Clone: 241158C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84119-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84119-3-RR against Human TRAPPC4 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HL-60, cellules MCF-7, cellules RAW 264.7, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HL-60,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84119-3-RR cible TRAPPC4 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRAPPC4 Protéine recombinante Ag2737
• Nom complet: trafficking protein particle complex 4
• Masse moléculaire calculée: 219 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC010866
• Symbole du gène: TRAPPC4
• Identification du gène (NCBI): 51399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

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