Anticorps Polyclonal de lapin anti-TRAPPC9/NIBP

TRAPPC9/NIBP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 19549-1-AP

Synonymes

IBP, IKBKBBP, KIAA1882, NIBP, NIK and IKBKB binding protein, T1, TRAPPC9, TRS120, Tularik gene 1 protein

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 19549-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19549-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 19549-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19549-1-AP (TRAPPC9/NIBP antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

19549-1-AP cible TRAPPC9/NIBP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: trafficking protein particle complex 9
• Masse moléculaire calculée: 139 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: NM_031466
• Symbole du gène: NIBP/Trappc9
• Identification du gène (NCBI): 83696
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRAPPC9, also named NIK- and IKBKB-binding protein (NIBP), is involved in the NF-kappaB signaling pathway and directly interacts with IKK-beta and MAP3K14. TRAPPC9 functions as an activator of NF-kappa-B via increased phosphorylation of the IKK complex. TARPPC9 may also function in neuronal cells differentiation and defects in TRAPPC9 are the cause of mental retardation autosomal recessive type 13 (MRT13), which is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior. TARPPC9 is a component of the multisubunit TRAPP (transport protein particle) complex and it may play a role in vesicular transport from endoplasmic reticulum to Golgi.