Anticorps Polyclonal de lapin anti-TRBP

• Phare
• Validé par KD/KO

TRBP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 15753-1-AP

Synonymes

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

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Galerie de données de validation

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  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15753-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 15753-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15753-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15753-1-AP (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15753-1-AP

  IP Result of anti-TRBP (IP:15753-1-AP, 4ug; Detection:15753-1-AP 1:1500) with mouse liver tissue lysate 6400ug.

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  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15753-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15753-1-AP (TRBP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15753-1-AP

  Immunofluorescent analysis of Hela cells, using TARBP2 antibody 15753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de souris, tissu splénique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IF: See 2 publications below
• IP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

15753-1-AP cible TRBP dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRBP Protéine recombinante Ag8255
• Nom complet: TAR (HIV-1) RNA binding protein 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: BC005860
• Symbole du gène: TARBP2
• Identification du gène (NCBI): 6895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Nuclear TARBP2 Drives Oncogenic Dysregulation of RNA Splicing and Decay.; Authors - Lisa Fish ; KD Validated
mouse	WB	Mol Cell; A Rapid Induction Mechanism for Lin28a in Trophic Responses.; Authors - Alexandra M Amen
human	WB	Nat Commun; Hypoxia regulates overall mRNA homeostasis by inducing Met1-linked linear ubiquitination of AGO2 in cancer cells.; Authors - Hailong Zhang
human	WB	Nat Commun; SUMOylation of TARBP2 regulates miRNA/siRNA efficiency.; Authors - Cheng Chen ; KD Validated
human	WB,IF	Nucleic Acids Res; S6K2-mediated regulation of TRBP as a determinant of miRNA expression in human primary lymphatic endothelial cells.; Authors - Matthew J Warner ; KD Validated
human	WB	J Med Chem; Identification of a Novel TAR RNA-Binding Protein 2 Modulator with Potential Therapeutic Activity against Hepatocellular Carcinoma.; Authors - Zongyuan Zhou