Anticorps Monoclonal anti-TRBP

• Phare
• Validé par KD/KO

TRBP Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C9A10

N° de cat : 66792-1-PBS

Synonymes

LOQS, TAR RNA binding protein 2, TARBP2, TRBP, TRBP1, TRBP2

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Galerie de données de validation

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  WB analysis using 66792-1-Ig (same clone as 66792-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66792-1-Ig (TRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66792-1-Ig (same clone as 66792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66792-1-Ig (TRBP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66792-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66792-1-PBS cible TRBP dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRBP Protéine recombinante Ag8269
• Nom complet: TAR (HIV-1) RNA binding protein 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: BC005860
• Symbole du gène: TRBP
• Identification du gène (NCBI): 6895
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TARBP2 is a component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC). Within the complex, TARBP2 and DICER1 are required to process precursor miRNAs (pre-miRNA) to mature miRNA and then load them onto EIF2C2/AGO2. It may has a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Otherwise, it binds to the HIV-1 TAR RNA that is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. The endogenous TRBP that was present in HEK293 cells, with apparent molecular masses of 43-46 kDa, are expressed in human cells. (PMID: 16142218)

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