Anticorps Recombinant de lapin anti-TREM2

TREM2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240384B7

N° de cat : 83438-6-PBS

Synonymes

240384B7, TREM 2, TREM-2, Trem2a, Trem2b

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Galerie de données de validation

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  WB analysis of THP-1 using 83438-6-RR (same clone as 83438-6-PBS)

  PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83438-6-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 83438-6-RR (same clone as 83438-6-PBS)

  Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83438-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83438-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83438-6-RR against Human TREM2 were performed. The affinity constant is 38.7 nM.

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Informations sur le produit

83438-6-PBS cible TREM2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TREM2 Protéine recombinante Eg0747
• Nom complet: triggering receptor expressed on myeloid cells 2
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 26-32 kDa
• Numéro d’acquisition GenBank: BC032362
• Symbole du gène: TREM2
• Identification du gène (NCBI): 54209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

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