Anticorps Recombinant de lapin anti-TREM2

TREM2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

240384B7

N° de cat : 83438-6-RR

Synonymes

240384B7, TREM 2, TREM-2, Trem2a, Trem2b

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Galerie de données de validation

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  WB analysis of THP-1 using 83438-6-RR

  PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 83438-6-RR

  Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 83438-6-RR (TREM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83438-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83438-6-RR against Human TREM2 were performed. The affinity constant is 38.7 nM.

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Applications testées

• Résultats positifs en WB: PMA treated THP-1 cells, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

83438-6-RR cible TREM2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TREM2 Protéine recombinante Eg0747
• Nom complet: triggering receptor expressed on myeloid cells 2
• Masse moléculaire calculée: 222 aa, 25 kDa
• Poids moléculaire observé: 26-32 kDa
• Numéro d’acquisition GenBank: BC032362
• Symbole du gène: TREM2
• Identification du gène (NCBI): 54209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; TREM2 alleviates sepsis-induced acute lung injury by attenuating ferroptosis via the SHP1/STAT3 pathway; Authors - Siyi Wu
mouse	WB	ACS Infect Dis; A Photoactivatable Free Mycolic Acid Probe to Investigate Mycobacteria-Host Interactions; Authors - Kingsley C Agu

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