Anticorps Monoclonal anti-TRIB3

TRIB3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2C10C7

N° de cat : 66702-1-Ig

Synonymes

2C10C7, C20orf97, Neuronal cell death-inducible putative kinase, NIPK, p65-interacting inhibitor of NF-kappa-B

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Galerie de données de validation

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  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66702-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 66702-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse hepatocytes using 66702-1-Ig

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 66702-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 66702-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66702-1-Ig (TRIB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules DU 145, cellules HepG2, cellules HUVEC, cellules L02, cellules LNCaP, cellules MCF-7, cellules PC-3, hépatocytes de souris, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

66702-1-Ig cible TRIB3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIB3 Protéine recombinante Ag25776
• Nom complet: tribbles homolog 3 (Drosophila)
• Masse moléculaire calculée: 358 aa, 40 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC027484
• Symbole du gène: TRIB3
• Identification du gène (NCBI): 57761
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).

Publications

Species	Application	Title
mouse	WB	JCI Insight; Mitochondrial and NAD+ metabolism predict recovery from acute kidney injury in a diverse mouse population; Authors - Jean-David Morel
human	WB	Phytomedicine; Cepharanthine sensitizes gastric cancer cells to chemotherapy by targeting TRIB3-FOXO3-FOXM1 axis to inhibit autophagy; Authors - Yang-Yang Lu
human	WB	Drug Des Devel Ther; PMID: 31118581; Authors - Hang Zhao

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