Anticorps Polyclonal de lapin anti-TRIM16

• Phare
• Validé par KD/KO

TRIM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 24403-1-AP

Synonymes

E3 ubiquitin-protein ligase TRIM16, EBBP, EC:2.3.2.27, Estrogen-responsive B box protein, tripartite motif containing 16

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Galerie de données de validation

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  WB analysis of HepG2 using 24403-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24403-1-AP (TRIM16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24403-1-AP

  IP result of anti-TRIM16 (IP:24403-1-AP, 4ug; Detection:24403-1-AP 1:300) with HeLa cells lysate 3960ug.

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 24403-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 24403-1-AP (TRIM16 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 24403-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 24403-1-AP (TRIM16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu testiculaire humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

24403-1-AP cible TRIM16 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM16 Protéine recombinante Ag21406
• Nom complet: tripartite motif-containing 16
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC015674
• Symbole du gène: TRIM16
• Identification du gène (NCBI): 10626
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902).

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2.; Authors - Yi Zhao ; KD Validated
human	WB,IHC	J Genet Genomics; RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma.; Authors - Leilei Zheng
human	WB	Exp Cell Res; TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.; Authors - Yi Zhao
human	WB,IHC	Exp Cell Res; TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.; Authors - Longhui Ruan ; KD Validated
human	WB	Cell Prolif; G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration; Authors - Shuai Li
human	WB,IF	Mol Carcinog; TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway; Authors - Shanfeng Li ; KD Validated

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