Anticorps Polyclonal de lapin anti-TRIM2

• Phare
• Validé par KD/KO

TRIM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 20356-1-AP

Synonymes

E3 ubiquitin-protein ligase TRIM2, EC:2.3.2.27, KIAA0517, RING-type E3 ubiquitin transferase TRIM2, RNF86

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Galerie de données de validation

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  WB analysis using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20356-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 20356-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 20356-1-AP (TRIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20356-1-AP

  IP result of anti-TRIM2 (IP:20356-1-AP, 4ug; Detection:20356-1-AP 1:300) with mouse brain tissue lysate 3200ug.

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  IHC staining of human kidney using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20356-1-AP (TRIM2 Antibody) at dilution of 1:50(under 40x lens).

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  IHC staining of mouse cerebellum using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20356-1-AP (TRIM2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20356-1-AP (TRIM2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20356-1-AP (TRIM2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 20356-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using TRIM2 antibody (20356-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu d'intestin grêle de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cervelet de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

20356-1-AP cible TRIM2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM2 Protéine recombinante Ag14208
• Nom complet: tripartite motif-containing 2
• Masse moléculaire calculée: 744 aa, 82 kDa
• Poids moléculaire observé: 70-85 kDa
• Numéro d’acquisition GenBank: BC011052
• Symbole du gène: TRIM2
• Identification du gène (NCBI): 23321
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Publications

Species	Application	Title
human	WB,IP,IF	Nat Commun; Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity; Authors - Diego Esposito
human	WB,IF	J Transl Med; Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.; Authors - Zili Zhou
human	WB	Cancer Cell Int; TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.; Authors - Zhaoxian Lin ; KD Validated
human	WB,IP,IF	FEBS J; TRIM2 promotes metabolic adaptation to glutamine deprivation via enhancement of CPT1A activity; Authors - Kaimin Liao
human	WB,IHC	Cell Mol Life Sci; E3 ubiquitin ligase TRIM2 identified as a novel suppressor of CYP11B2 and aldosterone production; Authors - Liang Chen ; KD Validated

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