Anticorps Polyclonal de lapin anti-TRIM25

• Phare
• Validé par KD/KO

TRIM25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(32)

Avis (1)

N° de cat : 12573-1-AP

Synonymes

EC:2.3.2.27, EC:6.3.2.n3, EFP, Estrogen-responsive finger protein, RING-type E3 ubiquitin transferase

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Galerie de données de validation

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  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12573-1-AP

  IP result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.

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  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM25 antibody (12573-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 33 publications below
• IF: See 10 publications below
• IP: See 5 publications below
• CoIP: See 3 publications below

Informations sur le produit

12573-1-AP cible TRIM25 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM25 Protéine recombinante Ag3241
• Nom complet: tripartite motif-containing 25
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 68-71 kDa
• Numéro d’acquisition GenBank: BC016924
• Symbole du gène: TRIM25
• Identification du gène (NCBI): 7706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

Publications

Species	Application	Title
	WB	Signal Transduct Target Ther; Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis; Authors - Yuanliang Yan
human	WB	Adv Sci (Weinh); Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy; Authors - Zhao-Xin Zhang
human	WB	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang
human	WB	Elife; Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.; Authors - Wei Liu
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer cells and tissue

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