Anticorps Polyclonal de lapin anti-TRIM25

• Phare
• Validé par KD/KO

TRIM25 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

23

Avis (1)

N° de cat : 12573-1-AP

Synonymes

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

---

Galerie de données de validation

• 

  WB analysis using 12573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12573-1-AP (TRIM25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12573-1-AP

  IP Result of anti-TRIM25 (IP:12573-1-AP, 4ug; Detection:12573-1-AP 1:600) with K-562 cells lysate 3200ug.

• 

  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12573-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12573-1-AP (TRIM25 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12573-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12573-1-AP (TRIM25 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, tissu hépatique de souris, tissu pancréatique de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

12573-1-AP cible TRIM25 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM25 Protéine recombinante Ag3241
• Nom complet: tripartite motif-containing 25
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 68-71 kDa
• Numéro d’acquisition GenBank: BC016924
• Symbole du gène: TRIM25
• Identification du gène (NCBI): 7706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity(PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection(PMID: 17392790). TRIM25 can be detected in both the cytoplasmic and nuclear (PMID: 33966039).

Publications

Species	Application	Title
human,mouse	WB	Nat Immunol; USP15 regulates type I interferon response and is required for pathogenesis of neuroinflammation; Authors - ; KO Validated
mouse	WB	Nat Immunol; Caspase-12 controls West Nile virus infection via the viral RNA receptor RIG-I.; Authors - Wang Penghua P
human	WB	Cell Rep; p53-responsive CMBL reprograms glucose metabolism and suppresses cancer development by destabilizing phosphofructokinase PFKP; Authors - Yingdan Huang
human	WB	Elife; Cyclophilin A-regulated ubiquitination is critical for RIG-I-mediated antiviral immune responses.; Authors - Wei Liu
human	WB	Haematologica; Hypoxia-induced long non-coding RNA DARS-AS1 regulates RBM39 stability to promote myeloma malignancy.; Authors - Jia Tong
human	WB	MBio; ISG15 Deficiency Enhances HIV-1 Infection by Accumulating Misfolded p53.; Authors - Edmund Osei Kuffour

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Iram (Verified Customer) (01-15-2021)

TRIM25 antibody giving a very clear and sharp expression.

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
• Cell Tissue Type: Human colon cancer cells and tissue