Anticorps Monoclonal anti-TRIM25

• Phare
• Validé par KD/KO

TRIM25 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1H6G1

N° de cat : 67314-1-Ig

Synonymes

EFP, RING finger protein 147, RNF147, TRIM25, tripartite motif containing 25, Z147, Zinc finger protein 147, ZNF147

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Galerie de données de validation

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  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67314-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67314-1-Ig (TRIM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 67314-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67314-1-Ig (TRIM25 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293T using 67314-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using TRIM25 antibody (67314-1-Ig, Clone: 1H6G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules COLO 320, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP, cellules THP-1
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293T,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

67314-1-Ig cible TRIM25 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIM25 Protéine recombinante Ag29325
• Nom complet: tripartite motif-containing 25
• Masse moléculaire calculée: 630 aa, 71 kDa
• Poids moléculaire observé: 68-71 kDa
• Numéro d’acquisition GenBank: BC016924
• Symbole du gène: TRIM25
• Identification du gène (NCBI): 7706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM25 (tripartite motif-containing 25) is also named as EFP, Z147, ZNF147, RNF147 and belongs to the zinc finger protein family and tripartite motif (TRIM) family. It induces the Lys63-linked ubiquitination of RIG-I, which is crucial for the cytosolic RIG-I signalling pathway to elicit host antiviral innate immunity (PMID:17392790). It is also essential not only for DDX58 ubiquitination but also for DDX58-mediated interferon-production and antiviral activity in response to RNA virus infection (PMID: 17392790).

Publications

Species	Application	Title
human	WB,CoIP	Elife; RTN3 inhibits RIG-I-mediated antiviral responses by impairing TRIM25-mediated K63-linked polyubiquitination.; Authors - Ziwei Yang ; KD Validated
human	WB	Exp Biol Med (Maywood); Tripartite motif-containing 25 facilitates immunosuppression and inhibits apoptosis of glioma via activating NF-κB.; Authors - Mao-Xu Ge ; KD Validated
human	IHC	Cell Death Dis; TRIM25 regulates oxaliplatin resistance in colorectal cancer by promoting EZH2 stability.; Authors - Sha Zhou
human	WB,IP	Oncogene; TRIM25 promotes temozolomide resistance in glioma by regulating oxidative stress and ferroptotic cell death via the ubiquitination of keap1; Authors - Jianwei Wei ; KD Validated