Anticorps Polyclonal de lapin anti-TRIM27

• Phare
• Validé par KD/KO

TRIM27 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

18

Avis (4)

N° de cat : 12205-1-AP

Synonymes

Ret finger protein, RFP, RING finger protein 76, RNF76, TRIM27, tripartite motif containing 27, Zinc finger protein RFP

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Galerie de données de validation

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  WB analysis of Jurkat using 12205-1-AP

  WB result of TRIM27 antibody (12205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM27 transfected Jurkat cells.

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  WB analysis using 12205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12205-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12205-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12205-1-AP

  IP Result of anti-TRIM27 (IP:12205-1-AP, 4ug; Detection:12205-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lung cancer using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 3 publications below

Informations sur le produit

12205-1-AP cible TRIM27 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM27 Protéine recombinante Ag2849
• Nom complet: tripartite motif-containing 27
• Masse moléculaire calculée: 513 aa, 58 kDa
• Poids moléculaire observé: 58 kDa, 41 kDa
• Numéro d’acquisition GenBank: BC013580
• Symbole du gène: TRIM27
• Identification du gène (NCBI): 5987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Elife; The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.; Authors - Zane A Gibbs
human	CoIP	Mol Ther Nucleic Acids; TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination.; Authors - Li Xing
human	WB	FASEB J; TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.; Authors - Yinfang Wang ; KD Validated
human	WB,IF	J Cell Mol Med; NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.; Authors - Bowen Hu
mouse	WB	J Leukoc Biol; Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes.; Authors - Tongjie Wang
human	WB,IF	FEBS J; TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1; Authors - Juncal Garcia‐garcia ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jason (Verified Customer) (08-17-2022)

Third time purchase. I am satisfied. This antibody works very well in human breast cancer cells using WB at dilution 1:1000, but many non-specific IF singling detected.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WB: 1:1000

FH

Elisa (Verified Customer) (12-21-2021)

HAP1 were lysates with RIPA buffer. 1mg of protein was loaded per condition. Proteins were run on a 12-4% gradient polyacrylamide gel at 180V constant. Gel was transferred on nitrocellulose filter at 300mA constant. Blots were then blocked in 5%milk in TBS-0,1%Tween for 30 min and then incubated with Trim27 primary antibody (1:1000 dilution). Anti-rabbit -HRP conjugated antibody (1:2000) was used to detect TRIM27 signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HAP1

TRIM27 Antibody Western Blot validation (1:1000 dilution) in HAP1 (Cat no:12205-1-AP)

FH

Sarah (Verified Customer) (07-03-2019)

Total cell lysate (30 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-TRIM27 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T

TRIM27 Antibody Western Blot, validation (1:1000 dilution) in HEK 293T (Cat no:12205-1-AP)

FH

Francesca (Verified Customer) (05-16-2019)

Excellent antibody, clean and strong signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: U2OS and A549 cells

TRIM27 Antibody Western Blot, validation (1:2000 dilution) in U2OS and A549 cells (Cat no:12205-1-AP)