Anticorps Recombinant de lapin anti-TRIM27

TRIM27 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242538H5

N° de cat : 84927-6-PBS

Synonymes

Tripartite motif-containing protein 27, TRIM, RNF76, RFP, EC:2.3.2.27

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Galerie de données de validation

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  IF Staining of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM27 antibody (84927-6-RR, Clone: 242538H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 84927-6-RR (same clone as 84927-6-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84927-6-RR (same clone as 84927-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Trim27 Recombinant Antibody (84927-6-RR, Clone:242538H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84927-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84927-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84927-6-RR against Human TRIM27 were performed. The affinity constant is 0.653 nM.

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Informations sur le produit

84927-6-PBS cible TRIM27 dans les applications de IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM27 Protéine recombinante Ag2849
• Nom complet: tripartite motif-containing 27
• Masse moléculaire calculée: 513 aa, 58 kDa
• Numéro d’acquisition GenBank: BC013580
• Symbole du gène: TRIM27
• Identification du gène (NCBI): 5987
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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