Anticorps Recombinant de lapin anti-TRIM29

TRIM29 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251298E11

N° de cat : 86496-1-RR

Synonymes

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

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Galerie de données de validation

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  WB analysis of HT-29 using 86496-1-RR

  HT-29 cells were subjected to SDS PAGE followed by western blot with 86496-1-RR (TRIM29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86496-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86496-1-RR (TRIM29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86496-1-RR against Human TRIM29 were performed. The affinity constant is 0.424 nM.

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules A431, cellules BGC-823, cellules HaCaT, cellules HT-29

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86496-1-RR cible TRIM29 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM29 Protéine recombinante Ag11600
• Nom complet: tripartite motif-containing 29
• Masse moléculaire calculée: 588 aa, 66 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC017352
• Symbole du gène: TRIM29
• Identification du gène (NCBI): 23650
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).

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