Anticorps Polyclonal de lapin anti-TRIM33

• Phare
• Validé par KD/KO

TRIM33 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55374-1-AP

Synonymes

Ectodermin homolog, KIAA1113, Protein Rfg7, PTC7, RET fused gene 7 protein, RFG7, TF1G, TIF1 gamma, TIF1G, TIF1GAMMA, TIFGAMMA, TRIM33, tripartite motif containing 33

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Galerie de données de validation

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  WB analysis of COLO 320 using 55374-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 55374-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 55374-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 55374-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55374-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

55374-1-AP cible TRIM33 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tripartite motif-containing 33
• Masse moléculaire calculée: 122 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_015906
• Symbole du gène: TRIM33
• Identification du gène (NCBI): 51592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB,IHC	Technol Cancer Res Treat; Downregulation of TRIM33 Promotes Survival and Epithelial-Mesenchymal Transition in Gastric Cancer; Authors - Fang Wang ; KD Validated
human	WB,IF,IP	Exp Dermatol; Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis; Authors - Luting Yang