Anticorps Polyclonal de lapin anti-TRIM33

• Phare
• Validé par KD/KO

TRIM33 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 55374-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of COLO 320 using 55374-1-AP (same clone as 55374-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 55374-1-AP (same clone as 55374-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 55374-1-AP (same clone as 55374-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55374-1-AP (TRIM33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 55374-1-AP (same clone as 55374-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55374-1-AP (TRIM33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55374-1-PBS in a different storage buffer formulation.

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Informations sur le produit

55374-1-PBS cible TRIM33 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tripartite motif-containing 33
• Masse moléculaire calculée: 122 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_015906
• Symbole du gène: TRIM33
• Identification du gène (NCBI): 51592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRIM33, also named as KIAA1113, RFG7 and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight of detected is about 140-150 kDa as the same as the result from the paper(PMID:25639486;26592194).

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