Anticorps Recombinant de lapin anti-TRIM33

TRIM33 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242728H2

N° de cat : 85364-3-RR

Synonymes

242728H2, E3 ubiquitin-protein ligase TRIM33, EC:2.3.2.27, Ectodermin homolog, KIAA1113

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Galerie de données de validation

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  WB analysis using 85364-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 85364-3-RR

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 85364-3-RR (TRIM33 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85364-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRIM33 antibody (85364-3-RR, Clone: 242728H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules COLO 320, cellules MOLT-4
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85364-3-RR cible TRIM33 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tripartite motif-containing 33
• Masse moléculaire calculée: 122 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: NM_015906
• Symbole du gène: TRIM33
• Identification du gène (NCBI): 51592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM33, also named KIAA1113, RFG7, and TIF1G, belongs to the TRIM/RBCC family. It acts as an E3 ubiquitin-protein ligase. TRIM33 promotes SMAD4 ubiquitination, nuclear exclusion, and degradation via the ubiquitin-proteasome pathway. It may act as a transcriptional repressor. It inhibits the transcriptional response to TGF-beta/BMP signaling cascade. TRIM33 plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3, TRIM33 stimulates erythroid differentiation of hematopoietic stem/progenitor. Defects in TRIM33 are a cause of thyroid papillary carcinoma (TPC). This antibody is specific to TRIM33. The calculated molecular weight of TRIM33 is 122kDa, but the molecular weight detected is about 140-150 kDa the same as the result from the paper(PMID:25639486;26592194).

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