Anticorps Recombinant de lapin anti-TRIM34

TRIM34 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240180E2

N° de cat : 83316-2-RR

Synonymes

IFP1, RING finger protein 21, RNF21, TRIM34, tripartite motif containing 34

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Galerie de données de validation

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  WB analysis of K-562 using 83316-2-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 83316-2-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83316-2-RR (TRIM34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 83316-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using TRIM34 antibody (83316-2-RR, Clone: 240180E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83316-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83316-2-RR against Human TRIM34 were performed. The affinity constant is 0.941 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu placentaire humain
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83316-2-RR cible TRIM34 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM34 Protéine recombinante Ag34444
• Nom complet: tripartite motif-containing 34
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 31 kDa, 57 kDa
• Numéro d’acquisition GenBank: NM_001003827
• Symbole du gène: TRIM34
• Identification du gène (NCBI): 53840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif 34 (TRIM34) is a member of TRIM family that can be highly induced by type I Interferon. TRIM34 has a stimulative role in cell apoptosis and a suppressive role in inflammation. TRIM34 serves a tumor suppressor role in the occurrence and development of colon cancer by enhancing cell apoptosis. TRIM34 possesses at least three kinds of isoforms: the long form at 97 kDa, medium form at 57 kDa, the short form 31 kDa (PMID: 11013086, 31956709).

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