Anticorps Polyclonal de lapin anti-TRIM35

• Phare
• Validé par KD/KO

TRIM35 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19806-1-PBS

Synonymes

E3 ubiquitin-protein ligase TRIM35, EC:2.3.2.27, HLS5, KIAA1098, tripartite motif containing 35

---

Galerie de données de validation

• 

  WB analysis of A431 using 19806-1-AP (same clone as 19806-1-PBS)

  WB result of TRIM35 antibody (19806-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM35 transfected A431 cells. This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19806-1-AP (same clone as 19806-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19806-1-AP (TRIM35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 19806-1-AP (same clone as 19806-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRIM35 antibody (19806-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19806-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

19806-1-PBS cible TRIM35 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM35 Protéine recombinante Ag13584
• Nom complet: tripartite motif-containing 35
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 45~50 kDa
• Numéro d’acquisition GenBank: BC018337
• Symbole du gène: TRIM35
• Identification du gène (NCBI): 23087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif 35 (TRIM35), also called hemopoietic lineage switch (Hls5), is a member of the RING finger, B box, coiled-coil (RBCC), or TRIM family E3 ligases expressed in a wide variety of hemopoietic cell types, including fetal liver progenitors. TRIM35 is reported to be a tumor suppressor gene and is expressed at low levels in several types of cancer (PMID: 34124276, 35081724). Western detected a band at 45-50 kDa possibly due to the high proline content.

{{ptg:RelatedPrimaryAntibodies}}