Anticorps Polyclonal de lapin anti-TRIM46

• Phare
• Validé par KD/KO

TRIM46 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21026-1-AP

Synonymes

Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

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Galerie de données de validation

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  WB analysis of mouse lung using 21026-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21026-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21026-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21026-1-AP (TRIM46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 21026-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21026-1-AP (TRIM46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21026-1-AP

  Immunofluorescent analysis of HepG2 cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 21026-1-AP

  Immunofluorescent analysis of Hela cells, using TRIM46 antibody 21026-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules HeLa, tissu testiculaire de souris
• Résultats positifs en IHC: tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

21026-1-AP cible TRIM46 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM46 Protéine recombinante Ag15290
• Nom complet: tripartite motif-containing 46
• Masse moléculaire calculée: 759 aa, 83 kDa
• Poids moléculaire observé: 55 kDa, 62 kDa
• Numéro d’acquisition GenBank: BC069416
• Symbole du gène: TRIM46
• Identification du gène (NCBI): 80128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IP	Nat Commun; Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.; Authors - John K Vuong
mouse	WB,IF	Cell Rep; The Spatiotemporal Construction of the Axon Initial Segment via KIF3/KAP3/TRIM46 Transport under MARK2 Signaling.; Authors - Sotaro Ichinose
human	IHC	Oncol Res; Tripartite Motif-Containing 46 Promotes Viability and Inhibits Apoptosis of Osteosarcoma Cells by Activating NF-B Signaling Through Ubiquitination of PPAR.; Authors - Wenwei Jiang
human	WB	Exp Cell Res; TRIM46 contributes to high glucose-induced ferroptosis and cell growth inhibition in human retinal capillary endothelial cells by facilitating GPX4 ubiquitination.; Authors - Jingfa Zhang ; KD Validated
human	WB,IF	Int J Mol Sci; Mmu-miR-1894-3p Inhibits Cell Proliferation and Migration of Breast Cancer Cells by Targeting Trim46.; Authors - Li Zhang
human	WB,CoIP	Eye Vis (Lond); TRIM46 aggravated high glucose-induced hyper permeability and inflammatory response in human retinal capillary endothelial cells by promoting IκBα ubiquitination; Authors - Hangqi Shen ; KO Validated