Anticorps Polyclonal de lapin anti-TRIM46

TRIM46 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 30298-1-AP

Synonymes

FLJ23229, Gene Y protein, GENEY, TRIFIC, TRIM46, tripartite motif containing 46

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Galerie de données de validation

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  WB analysis using 30298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30298-1-AP (TRIM46 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 30298-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TRIM46 antibody (30298-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

30298-1-AP cible TRIM46 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM46 Protéine recombinante Ag33175
• Nom complet: tripartite motif-containing 46
• Masse moléculaire calculée: 759 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC069416
• Symbole du gène: TRIM46
• Identification du gène (NCBI): 80128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

Publications

Species	Application	Title
mouse	WB	J Cell Sci; Efficient axonal transport of endolysosomes relies on the balanced ratio of microtubule tyrosination and detyrosination; Authors - Anja Konietzny
human	WB	J Bioenerg Biomembr; TRIM46 accelerates H1N1 influenza virus-induced ferroptosis and inflammatory response by regulating SLC7A11 ubiquitination; Authors - Chao Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yannes (Verified Customer) (04-08-2024)

works well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: rat brain lysate, mouse primary hippocampal neurons

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