Anticorps Recombinant de lapin anti-TRIM46

TRIM46 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240557E1

N° de cat : 83614-2-PBS

Synonymes

240557E1, GeneY, TRIFIC, tripartite motif containing 46, Tripartite motif-containing protein 46

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Galerie de données de validation

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  WB analysis using 83614-2-RR (same clone as 83614-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 83614-2-RR (same clone as 83614-2-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 83614-2-RR (TRIM46 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83614-2-RR (TRIM46 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 83614-2-RR (same clone as 83614-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83614-2-RR (same clone as 83614-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRIM46 antibody (83614-2-RR, Clone: 240557E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83614-2-RR (same clone as 83614-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRIM46 Recombinant antibody (83614-2-RR, Clone:240557E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83614-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00591-1

  Cytometric bead array standard curve of MP00591-1, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-4-PBS. Standard: Ag15290. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP00591-2

  Cytometric bead array standard curve of MP00591-2, TRIM46 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83614-2-PBS. Detection antibody: 83614-3-PBS. Standard: Ag15290. Range: 1.25-40 ng/mL.

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  Affinity and Kinetic Characterization of 83614-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83614-2-RR against Human TRIM46 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83614-2-PBS cible TRIM46 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM46 Protéine recombinante Ag15290
• Nom complet: tripartite motif-containing 46
• Masse moléculaire calculée: 759 aa, 83 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC069416
• Symbole du gène: TRIM46
• Identification du gène (NCBI): 80128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing protein 46 (TRIM46), a microtubule-associated protein, is involved in the formation of parallel microtubule bundles linked by cross-bridges in the proximal axon. Required for the uniform orientation and maintenance of the parallel microtubule fascicles, which are important for efficient cargo delivery and trafficking in axons. Thereby also required for proper axon specification, the establishment of neuronal polarity, and proper neuronal migration. The TRIM46 protein reported in previous studies includes the long isoform TRIM46L (83 kDa) and the short isoform TRIM46S (60 kDa).

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