Anticorps Polyclonal de lapin anti-TRIM47

• Phare
• Validé par KD/KO

TRIM47 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 26885-1-AP

Synonymes

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

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Galerie de données de validation

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  WB analysis using 26885-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26885-1-AP (TRIM47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26885-1-AP

  IP result of anti-TRIM47 (IP:26885-1-AP, 4ug; Detection:26885-1-AP 1:300) with U2OS cells lysate 2000ug.

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  IHC staining of human colon cancer using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26885-1-AP (TRIM47 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of rat kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 26885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 26885-1-AP (TRIM47 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules U2OS, tissu de côlon de rat, tissu de côlon de souris
• Résultats positifs en IP: cellules U2OS
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon de souris, tissu rénal de rat, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

26885-1-AP cible TRIM47 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM47 Protéine recombinante Ag22868
• Nom complet: tripartite motif-containing 47
• Masse moléculaire calculée: 638 aa, 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC017299
• Symbole du gène: TRIM47
• Identification du gène (NCBI): 91107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis.

Publications

Species	Application	Title
human	WB,IHC	Neoplasma; Trim47 overexpression correlates with poor prognosis in gastric cancer.; Authors - YuJian Xia
mouse,human	WB,IP,IHC	Signal Transduct Target Ther; TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2.; Authors - Yisong Qian ; KD Validated
human	WB,IHC	In Vitro Cell Dev Biol Anim; TRIM47 promotes glioma angiogenesis by suppressing Smad4; Authors - Zifan Wang ; KD Validated
mouse,human	WB,IHC,IF	Clinics (Sao Paulo); TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling; Authors - Xi Wang ; KD Validated
rat	WB	Exp Brain Res; Knocking down Trim47 ameliorated sevoflurane-induced neuronal cell injury and cognitive impairment in rats; Authors - Yingjun Zhu ; KD Validated
human	WB,IF	Comput Struct Biotechnol J; Featured interactome of homocysteine-inducible endoplasmic reticulum protein uncovers novel binding partners in response to ER stress; Authors - Rui Su

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