Anticorps Recombinant de lapin anti-TRIM47

TRIM47 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251436A2

N° de cat : 86728-1-RR

Synonymes

E3 ubiquitin-protein ligase TRIM47, EC:2.3.2.27, GOA, RNF100, tripartite motif containing 47

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Galerie de données de validation

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  WB analysis using 86728-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of OVCAR-3 using 86728-1-RR

  OVCAR-3 cells were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86728-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86728-1-RR (TRIM47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, cellules SMMC-7721, cellules U2OS, tissu de côlon de rat, tissu de côlon de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86728-1-RR cible TRIM47 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM47 Protéine recombinante Ag22868
• Nom complet: tripartite motif-containing 47
• Masse moléculaire calculée: 638 aa, 70 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC017299
• Symbole du gène: TRIM47
• Identification du gène (NCBI): 91107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM47/GOA is a member of the tripartite motif RBCC/Trim domain family that contains a RING-finger domain, B-boxes, and an alpha-helical coiled-coil region. TRIM47/GOA was identified as a protein overexpressed in astrocytoma tumor cells and is thought to play a role in dedifferentiation associated with tumorigenesis. TRIM47, defined as E3 ubiquitin ligases, physically binds to SMAD4 and enhances the ubiquitination and degradation of SMAD4 (PMID: 39117713, 35513381).

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