Anticorps Recombinant de lapin anti-TRIM56

TRIM56 Recombinant Antibody for WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242497B4

N° de cat : 84896-5-PBS

Synonymes

E3 ubiquitin-protein ligase TRIM56, EC:2.3.2.27, RNF109, TRIM, tripartite motif containing 56

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Galerie de données de validation

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  WB analysis using 84896-5-RR (same clone as 84896-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84896-5-RR (TRIM56 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84896-5-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01657-3

  Sandwich ELISA standard curve of MP01657-3, Human TRIM56 Recombinant Matched Antibody Pair - PBS only. 84896-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag21992. 84896-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP01657-3

  The mean TRIM56 concentration was determined to be 34.62 ng/mL in K-562 cell extract based on a 2.4 mg/mL extract load, 38.07 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 40.37 ng/mL in A549 cell extract based on a 2.0 mg/mL extract load.

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  IHC staining of mouse kidney using 84896-5-RR (same clone as 84896-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84896-5-RR (TRIM56 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84896-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84896-5-RR (same clone as 84896-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84896-5-RR (TRIM56 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84896-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84896-5-RR (same clone as 84896-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRIM56 antibody (84896-5-RR, Clone: 242497B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84896-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84896-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84896-5-RR against Human TRIM56 were performed. The affinity constant is 7.59 nM.

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Informations sur le produit

84896-5-PBS cible TRIM56 dans les applications de WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM56 Protéine recombinante Ag21992
• Nom complet: tripartite motif-containing 56
• Masse moléculaire calculée: 755 aa, 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC048194
• Symbole du gène: TRIM56
• Identification du gène (NCBI): 81844
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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