Anticorps Polyclonal de lapin anti-TRIM62

TRIM62 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15256-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 15256-1-AP (same clone as 15256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15256-1-AP (TRIM62 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 15256-1-AP (same clone as 15256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 15256-1-AP (TRIM62 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15256-1-AP (same clone as 15256-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TRIM62 antibody (15256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15256-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15256-1-PBS cible TRIM62 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM62 Protéine recombinante Ag7143
• Nom complet: tripartite motif-containing 62
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC001222
• Symbole du gène: TRIM62
• Identification du gène (NCBI): 55223
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRIM62(Tripartite motif-containing protein 62) belongs to the TRIM/RBCC family. TRIM proteins are involved in a broad range of biological processes, including some that underlie genetic disorders, neurological disorders and cancers. TRIM62 is shown to promote the release of HIV from infected cells, and a TRIM62 protein that has a mutation in its RING domain was more active than the native protein in promoting HIV release(PMID:18836477).

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