Anticorps Polyclonal de lapin anti-TRIM65

TRIM65 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31990-1-PBS

Synonymes

E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, Tripartite motif-containing protein 65

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Galerie de données de validation

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  WB analysis using 31990-1-AP (same clone as 31990-1-PBS)

  K-562 cells lysate was subjected to SDS PAGE followed by western blot with 31990-1-AP (TRIM65 antibody) at dilution of 1:1000 incubated at room temperature for 1 hour. This data was developed using the same antibody clone with 31990-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31990-1-PBS cible TRIM65 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM65 Protéine recombinante Ag34639
• Nom complet: tripartite motif-containing 65
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57 kDa, 70 kDa
• Numéro d’acquisition GenBank: NM_173547
• Symbole du gène: TRIM65
• Identification du gène (NCBI): 201292
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tripartite motif-containing protein 65 (TRIM65) is a member of the TRIM family of RING-type E3 ubiquitin ligases. It contains a RING-finger domain, a B-box domain, a coiled-coil domain, and a SPRY domain (PMID: 40473847). As an E3 ubiquitin ligase, TRIM65 is involved in the ubiquitination of substrate proteins, playing a role in various intracellular physiological processes, including intracellular signaling, protein degradation, cell proliferation, apoptosis, carcinogenesis, autophagy, and phenotypic transformation (PMID: 38939997). TRIM65 has been shown to promote ubiquitination and degradation of several proteins, such as TOX4, which is involved in apoptosis, and ARHGAP35, which is involved in cell migration (PMID: 38212319; 31332286). The 70 kDa band might be the result of SUMO modification.

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