Anticorps Polyclonal de lapin anti-TRIM69

• Phare
• Validé par KD/KO

TRIM69 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 12951-1-AP

Synonymes

HSD 34, HSD34, RING finger protein 36, RNF36, Trif, TRIM69, tripartite motif containing 69

---

Galerie de données de validation

• 

  WB analysis of mouse testis using 12951-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12951-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12951-1-AP (TRIM69 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12951-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 12951-1-AP (TRIM69 antibody) at dilution of 1:100 (under 10x lens).

---

Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa
• Résultats positifs en IHC: tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

12951-1-AP cible TRIM69 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM69 Protéine recombinante Ag4074
• Nom complet: tripartite motif-containing 69
• Masse moléculaire calculée: 500 aa, 57 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC033314
• Symbole du gène: TRIM69
• Identification du gène (NCBI): 140691
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E3 ubiquitin-protein ligase TRIM69 (TRIM69) plays an important role in antiviral immunity by restricting different viral infections including dengue virus or vesicular stomatitis Indiana virus (PubMed:31375575, PubMed:31578292). It plays also a role in cataract formation together with TP53 (PubMed:30844644). Mechanistically, inhibits UVB-induced cell apoptosis and reactive oxygen species (ROS) production by inducing TP53 ubiquitination (PubMed:30844644). It has 4 isoforms with molecular weights of 57, 39, 34, and 32 kDa.

Publications

Species	Application	Title
human	WB,IHC,IF	Oncogene; TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD; Authors - Linqing Sun ; KD Validated
Human,monkey	WB	Front Immunol; DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection.; Authors - Huiyuan Jing
human	WB	Cell Physiol Biochem; Radioprotective Effects of Heat-Killed Mycobacterium Tuberculosis in Cultured Cells and Radiosensitive Tissues.; Authors - Yuanyuan Chen
human	WB	J Virol; Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State.; Authors - Tonya Kueck
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
mouse	IF, WB	J Biomed Res; The testis-specifically expressed gene Trim69 is not essential for fertility in mice.; Authors - Xi He ; KO Validated

{{ptg:RelatedPrimaryAntibodies}}