Anticorps Polyclonal de lapin anti-TRIM72

• Phare
• Validé par KD/KO

TRIM72 Polyclonal Antibody for WB, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 22151-1-AP

Synonymes

tripartite motif containing 72, TRIM, Mitsugumin-53, Mitsugumin 53, Mg53

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Galerie de données de validation

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  WB analysis using 22151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22151-1-AP (TRIM72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22151-1-AP

  IP result of anti-TRIM72 (IP:22151-1-AP, 4ug; Detection:22151-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skeletal muscle using 22151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using TRIM72 antibody (22151-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris,
• Résultats positifs en IF-P: tissu de muscle squelettique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

22151-1-AP cible TRIM72 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM72 Protéine recombinante Ag17756
• Nom complet: tripartite motif-containing 72
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC033211
• Symbole du gène: TRIM72
• Identification du gène (NCBI): 493829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF	Int J Mol Sci; PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice; Authors - Baile Wu
mouse	WB	Int Immunopharmacol; MG53 binding to CAV3 facilitates activation of eNOS/NO signaling pathway to enhance the therapeutic benefits of bone marrow-derived mesenchymal stem cells in diabetic wound healing; Authors - Junwei Wu
human	WB,IP	Cancer Biomark; Ubiquitination and degradation of MGMT by TRIM72 increases the sensitivity of uveal melanoma cells to Dacarbazine treatment; Authors - Xun Li ; KD Validated
rat	WB	Biochem Biophys Res Commun; Mesenchymal stem cells-derived exosomes prevent sepsis-induced myocardial injury by a CircRTN4/miR-497-5p/MG53 pathway.; Authors - Jiang Li
rat	WB	BMC Cardiovasc Disord; Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner.; Authors - Jiawei Zhuang
human	WB	FASEB J; Chorionic villus-derived mesenchymal stem cells induce E3 ligase TRIM72 expression and regulate cell behaviors through ubiquitination of p53 in trophoblasts; Authors - Yan Li ; KD Validated

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