Anticorps Monoclonal anti-TRIM72

TRIM72 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris

Applications

WB, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

5F2C11

N° de cat : 67403-1-PBS

Synonymes

hMG53, Mg53, Mitsugumin 53, Mitsugumin-53, TRIM

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Galerie de données de validation

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  WB analysis using 67403-1-Ig (same clone as 67403-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67403-1-Ig (same clone as 67403-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67403-1-Ig (same clone as 67403-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67403-1-Ig (TRIM72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse skeletal muscle using 67403-1-Ig (same clone as 67403-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TRIM72 antibody (67403-1-Ig, Clone: 5F2C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

• 

  FC experiment of C2C12 using 67403-1-Ig (same clone as 67403-1-PBS)

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human TRIM72 (67403-1-Ig, Clone:5F2C11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67403-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67403-1-PBS cible TRIM72 dans les applications de WB, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, souris

• Réactivité: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIM72 Protéine recombinante Ag2560
• Nom complet: tripartite motif-containing 72
• Masse moléculaire calculée: 477 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC033211
• Symbole du gène: TRIM72
• Identification du gène (NCBI): 493829
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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