Anticorps Polyclonal de lapin anti-TRIM8

• Phare
• Validé par KD/KO

TRIM8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 27463-1-AP

Synonymes

RNF27, RING-type E3 ubiquitin transferase TRIM8, Glioblastoma-expressed RING finger protein, GERP, EC:2.3.2.27

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Galerie de données de validation

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  WB analysis of L02 using 27463-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27463-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27463-1-AP (TRIM8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 27463-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27463-1-AP (TRIM8 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

27463-1-AP cible TRIM8 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIM8 Protéine recombinante Ag26517
• Nom complet: tripartite motif-containing 8
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC021925
• Symbole du gène: TRIM8
• Identification du gène (NCBI): 81603
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).

Publications

Species	Application	Title
human	WB,IF,IP	Oncogene; Mutual regulation between TRIM21 and TRIM8 via K48-linked ubiquitination; Authors - Lin Wang ; KD Validated
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu
human	WB,IF	Cancer Med; TRIM8 promotes ovarian cancer proliferation and migration by targeting VDAC2 for ubiquitination and degradation; Authors - Fei Wu ; KD Validated
human	WB,IHC	J Orthop Surg Res; The prognostic value of ubiquitin/ubiquitin-like-related genes along with immune cell infiltration and clinicopathological features in osteosarcoma; Authors - Jian Wen
human	WB	Exp Mol Pathol; Down-regulation of long noncoding RNA LINC00472 alleviates sepsis-induced acute hepatic injury by regulating miR-373-3p/TRIM8 axis.; Authors - Lei Li
human	WB	Adipocyte; Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway; Authors - Mingxue Zhu ; KD Validated

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