Anticorps Recombinant de lapin anti-TRIM8

TRIM8 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242501B6

N° de cat : 85169-1-RR

Synonymes

RING-type E3 ubiquitin transferase TRIM8, Glioblastoma-expressed RING finger protein, GERP, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM8

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Galerie de données de validation

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  WB analysis using 85169-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85169-1-RR (TRIM8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85169-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85169-1-RR against Human TRIM8 were performed. The affinity constant is 0.647 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HCT 116, cellules HepG2, cellules MCF-7, cellules NIH/3T3, cellules PC-12

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85169-1-RR cible TRIM8 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRIM8 Protéine recombinante Ag26517
• Nom complet: tripartite motif-containing 8
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC021925
• Symbole du gène: TRIM8
• Identification du gène (NCBI): 81603
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIM8 (Tripartite motif containing protein 8), also known as RNF27 (RING finger protein 27), a member of TRIM family proteins, is known to play a dual role as both tumor suppressor and oncogene, and to function at the crosstalk of cancer and innate immunity. TRIM8 can function at two subcellular sites-nucleus and cytoplasm-to regulate NF-κB, one of the central signaling pathways that plays a critical role in carcinogenesis and inflammatory diseases (PMID: 33230447, 22262183). TRIM8 is composed of 551 amino acids (aa) with a molecular weight of 61.5 kDa, contains a Nuclear Localization Signal (NLS), and forms specific nuclear structures similar to the TRIM19/PML Nuclear Bodies (NBs) (PMID: 33807506, 29182544).

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