Anticorps Monoclonal anti-TRIM9

TRIM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2B10

N° de cat : 67268-1-PBS

Synonymes

KIAA0282, RING finger protein 91, RNF91, SPRING, TRIM9, tripartite motif containing 9

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Galerie de données de validation

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  WB analysis using 67268-1-Ig (same clone as 67268-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67268-1-Ig (TRIM9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 67268-1-Ig (same clone as 67268-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67268-1-Ig (TRIM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67268-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67268-1-PBS cible TRIM9 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIM9 Protéine recombinante Ag27856
• Nom complet: tripartite motif-containing 9
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 90 kDa, 79 kDa, 61 kDa
• Numéro d’acquisition GenBank: BC013414
• Symbole du gène: TRIM9
• Identification du gène (NCBI): 114088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRIM9(E3 ubiquitin-protein ligase TRIM9) is also named as RNF91 and belongs to the TRIM/RBCC family. TRIM9 protein is a brain-specific E3 ubiquitin ligase. Importantly, TRIM9 is present in LBs found in DLB and PD, suggesting that TRIM9 not only plays roles in the regulation of neuronal functions, but also participates in the formation or breakdown of abnormal inclusions through its ligase activity and besides the striatum and hippocampus, the highest expression of TRIM9 protein is seen in the frontal, temporal and occipital cortices through the western blot (PMID:20085810). It has been reported that TRIM9 is highly expressed in the cerebral cortex in mouse and human brains and that TRIM9 is decreased in damaged brains in patients who have Parkinson disease and dementia with Lewy bodies(PMID:22337885). It has 3 isoforms with molecular mass of 79, 90 and 61 kDa.

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