Anticorps Monoclonal anti-TRIP13

• Phare
• Validé par KD/KO

TRIP13 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1F1E9

N° de cat : 67759-1-Ig

Synonymes

16E1 BP, 16E1BP, TR interacting protein 13, TRIP 13, TRIP13

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Galerie de données de validation

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  WB analysis using 67759-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67759-1-Ig (TRIP13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67759-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67759-1-Ig (TRIP13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules HeLa, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP, cellules NIH/3T3, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

67759-1-Ig cible TRIP13 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRIP13 Protéine recombinante Ag6595
• Nom complet: thyroid hormone receptor interactor 13
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC000404
• Symbole du gène: TRIP13
• Identification du gène (NCBI): 9319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRIP13 gene encodes thyroid receptor-interacting protein 13, that interact with thyroid hormone receptors, also known as human papillomavirus type 16 E1 protein-binding protein (16E1BP). This protein belongs to the AAA ATPase family and the PCH2 subfamily. TRIP13 gene may be one of the several playing roles in early-stage non-small cell lung cancer. TRIP13 also functions in chromosome recombination and chromosome structure development during meiosis, and it is required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation.

Publications

Species	Application	Title
human	WB	J Adv Res; Tetrahydrocurcumin targets TRIP13 inhibiting the interaction of TRIP13/USP7/c-FLIP to mediate c-FLIP ubiquitination in triple-negative breast cancer; Authors - Yu-Jie Sun
human	WB	Environ Toxicol; TRIP13 exerts a cancer-promoting role in cervical cancer by enhancing Wnt/β-catenin signaling via ACTN4; Authors - Xiaoying Liu ; KD Validated
human,mouse	WB,IHC	ACS Omega; TRIP13 Plays an Important Role in the Sensitivity of Leukemia Cell Response to Sulforaphane Therapy; Authors - Lei Liu

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