Anticorps Polyclonal de lapin anti-TRIP4

TRIP4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12324-1-PBS

Synonymes

ASC 1, ASC-1, HsT17391, RQT4, Thyroid receptor-interacting protein 4

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Galerie de données de validation

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  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 12324-1-AP (same clone as 12324-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12324-1-AP (same clone as 12324-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 12324-1-AP (same clone as 12324-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12324-1-AP (TRIP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 12324-1-AP (same clone as 12324-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12324-1-AP (TRIP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12324-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12324-1-PBS cible TRIP4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRIP4 Protéine recombinante Ag2987
• Nom complet: thyroid hormone receptor interactor 4
• Masse moléculaire calculée: 581 aa, 66 kDa
• Poids moléculaire observé: 60-65 kDa
• Numéro d’acquisition GenBank: BC012448
• Symbole du gène: TRIP4
• Identification du gène (NCBI): 9325
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Thyroid hormone receptor interactor 4(TRIP4), other named ASC1, is a transcriptional coactivator that promote transcriptional efficiencies. It harbors an autonomous transactivation domain that contains a putative zinc finger motif, which serves as a binding site for nuclear receptors for thyroid, estrogen, all-trans-retinoic acid and RXR by interaction with SRC-1 or CBP/p300. There are two forms of ASC1 have been detected in mRNA level, isoform1(581aa, ~65kd) and isoform2(539aa,~60kd)(12077347,12390891)

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