Anticorps Polyclonal de lapin anti-TRMT1

TRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14970-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 14970-1-AP (same clone as 14970-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14970-1-AP (TRMT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14970-1-AP (same clone as 14970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 14970-1-AP (same clone as 14970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14970-1-AP (TRMT1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14970-1-AP (same clone as 14970-1-PBS)

  Immunofluorescent analysis of HeLa cells using 14970-1-AP (TRMT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14970-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14970-1-PBS cible TRMT1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRMT1 Protéine recombinante Ag6837
• Nom complet: TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC002492
• Symbole du gène: TRMT1
• Identification du gène (NCBI): 55621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

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