Anticorps Recombinant de lapin anti-TRMT1

TRMT1 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250178D5

N° de cat : 85897-5-PBS

Synonymes

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

---

Galerie de données de validation

• 

  WB analysis using 85897-5-RR (same clone as 85897-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 85897-5-RR (same clone as 85897-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85897-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85897-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

---

Informations sur le produit

85897-5-PBS cible TRMT1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRMT1 Protéine recombinante Ag6837
• Nom complet: TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC002492
• Symbole du gène: TRMT1
• Identification du gène (NCBI): 55621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

{{ptg:RelatedPrimaryAntibodies}}