Anticorps Recombinant de lapin anti-TRMT1

TRMT1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250178D5

N° de cat : 85897-5-RR

Synonymes

EC:2.1.1.216, TRM1, TRMT 1, tRNA (guanine(26)-N(2))-dimethyltransferase, tRNA 2,2-dimethylguanosine-26 methyltransferase

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Galerie de données de validation

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  WB analysis using 85897-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85897-5-RR (TRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 85897-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT1 antibody (85897-5-RR, Clone: 250178D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85897-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85897-5-RR against Human TRMT1 were performed. The affinity constant is 26.2 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HCT 116, cellules HEK-293T, cellules HepG2, cellules K-562, tissu testiculaire de rat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85897-5-RR cible TRMT1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRMT1 Protéine recombinante Ag6837
• Nom complet: TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC002492
• Symbole du gène: TRMT1
• Identification du gène (NCBI): 55621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRMT1 is an RNA methyltransferase responsible for the formation of N2,N2-dimethylguanosine (m2,2G) at position 26 in both cytosolic and mitochondrial tRNAs.TRMT1-catalyzed tRNA modifications are important for maintenance of global protein translation levels including proteins involved in cellular growth, development, and stress response. Human neural stem cells with TRMT1 knockdowns were found to have hypersensitivity to redox stress, implicating TRMT1 and the m2,2G26 modification in the regulation of redox homeostasis.

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