Anticorps Polyclonal de lapin anti-TRMT10C

• Phare
• Validé par KD/KO

TRMT10C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 29087-1-AP

Synonymes

RG9MTD1, MRPP1

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Galerie de données de validation

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  WB analysis using 29087-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29087-1-AP (TRMT10C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 29087-1-AP

  WB result of TRMT10C antibody (29087-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT10C transfected HepG2 cells.

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  IHC staining of human colon cancer using 29087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29087-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRMT10C antibody (29087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules PC-12, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

29087-1-AP cible TRMT10C dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRMT10C Protéine recombinante Ag30642
• Nom complet: RNA (guanine-9-) methyltransferase domain containing 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_017819
• Symbole du gène: TRMT10C
• Identification du gène (NCBI): 54931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).

Publications

Species	Application	Title
human	WB	Cell Death Discov; N1-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction; Authors - Zhangyang Qi
mouse	WB	Circulation; Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation; Authors - Matthew A Walker
mouse,human	WB	Phytother Res; Astragaloside IV protects renal tubular epithelial cells against oxidative stress-induced injury by upregulating CPT1A-mediated HSD17B10 lysine succinylation in diabetic kidney disease; Authors - Meng Wang
mouse	WB	BMC Cardiovasc Disord; Single-cell transcriptomics reveals writers of RNA modification-mediated immune microenvironment and cardiac resident Macro-MYL2 macrophages in heart failure; Authors - Yao-Lin Yang
	WB	Cancer Lett; YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer; Authors - Hongyu Chen

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