Anticorps Polyclonal de lapin anti-TRMT10C

• Phare
• Validé par KD/KO

TRMT10C Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29087-1-PBS

Synonymes

RG9MTD1, MRPP1

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Galerie de données de validation

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  WB analysis using 29087-1-AP (same clone as 29087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29087-1-AP (TRMT10C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 29087-1-AP (same clone as 29087-1-PBS)

  WB result of TRMT10C antibody (29087-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT10C transfected HepG2 cells. This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29087-1-AP (same clone as 29087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29087-1-AP (same clone as 29087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29087-1-AP (TRMT10C antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29087-1-AP (same clone as 29087-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TRMT10C antibody (29087-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29087-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29087-1-PBS cible TRMT10C dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRMT10C Protéine recombinante Ag30642
• Nom complet: RNA (guanine-9-) methyltransferase domain containing 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: NM_017819
• Symbole du gène: TRMT10C
• Identification du gène (NCBI): 54931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

tRNA methyltransferase 10 homolog C (TRMT10C, also known as MRPP1) is involved in mitochondrial RNA processing and modification (PMID: 21593607). It is essential for the 5'-ends processing of mitochondrial tRNAs and plays a critical role in mitochondrial respiration and translation (PMID: 18984158; 29040705). Mutations in TRMT10C may be a cause of mitochondrial disease and highlight the importance of RNA processing for correct mitochondrial function (PMID: 27132592).

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