Anticorps Polyclonal de lapin anti-TRMT6

• Phare
• Validé par KD/KO

TRMT6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16727-1-PBS

Synonymes

tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6, TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09

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Galerie de données de validation

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  WB analysis using 16727-1-AP (same clone as 16727-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16727-1-AP (TRMT6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 16727-1-AP (same clone as 16727-1-PBS)

  WB result of TRMT6 antibody (16727-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16727-1-AP (same clone as 16727-1-PBS)

  IP result of anti-TRMT6 (IP:16727-1-AP, 4ug; Detection:16727-1-AP 1:700) with HeLa cells lysate 2000 ug. This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16727-1-AP (same clone as 16727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16727-1-AP (TRMT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16727-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16727-1-PBS cible TRMT6 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRMT6 Protéine recombinante Ag10176
• Nom complet: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC001262
• Symbole du gène: TRMT6
• Identification du gène (NCBI): 51605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA. TRMT6 exists as three alternatively spliced isoforms that, in response to DNA damage, may be phosphorylated by ATM or ATR. This antibody is specific to 56kd TRMT6 protein.

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