Anticorps Recombinant de lapin anti-TRMT6

• Phare
• Validé par KD/KO

TRMT6 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240911C5

N° de cat : 83843-5-PBS

Synonymes

TRMT 6, TRM6, mRNA methyladenosine-N(1)-methyltransferase non-catalytic subunit TRM6, CGI-09, CGI 09

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Galerie de données de validation

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  WB analysis using 83843-5-RR (same clone as 83843-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83843-5-RR (TRMT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 83843-5-RR (same clone as 83843-5-PBS)

  WB result of TRMT6 antibody (83843-5-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRMT6 transfected HepG2 cells. This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83843-5-RR (same clone as 83843-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83843-5-RR (TRMT6 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83843-5-RR (same clone as 83843-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83843-5-RR (same clone as 83843-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TRMT6 antibody (83843-5-RR, Clone: 240911C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83843-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83843-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83843-5-RR against Human TRMT6 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83843-5-PBS cible TRMT6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRMT6 Protéine recombinante Ag10176
• Nom complet: tRNA methyltransferase 6 homolog (S. cerevisiae)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC001262
• Symbole du gène: TRMT6
• Identification du gène (NCBI): 51605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transfer RNA (tRNA) modifications help regulate the efficiency of mRNA translation by maintaining the correct reading frames within mRNA. TRMT6 (tRNA methyltransferase 6 ), also known as GCD10 or TRM6, is a 497 amino acid protein that localizes to the nucleus and exists as a heterodimer with TRM61. Expressed in liver, brain, ovary and testis, TRMT6 functions as a substrate-binding subunit of tRNA and is thought to catalyze the formation of N(1)-methyladenine at position 58 in initiator methionyl-tRNA.

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