Anticorps Recombinant de lapin anti-TRMU

TRMU Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250434A4

N° de cat : 86095-1-PBS

Synonymes

EC:2.8.1.14, Mitochondrial tRNA-specific 2-thiouridylase 1, MTO2, MTO2 homolog, MTU1

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Galerie de données de validation

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  WB analysis using 86095-1-RR (same clone as 86095-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86095-1-RR (TRMU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86095-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 86095-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86095-1-RR against Human TRMU were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86095-1-PBS cible TRMU dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRMU Protéine recombinante Ag21930
• Nom complet: tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
• Masse moléculaire calculée: 421 aa, 48 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC027991
• Symbole du gène: TRNT1
• Identification du gène (NCBI): 55687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRMU (tRNA m5C methyltransferase) is a mitochondrial protein involved in the modification of mitochondrial tRNA. Specifically, it catalyzes the formation of 5-methylcytidine at position 34 (m5C34) in the anticodon stem-loop of mitochondrial tRNAs, which is crucial for maintaining the stability and function of these tRNAs. TRMU is a nuclear-encoded protein located in the mitochondrial matrix. It plays a vital role in mitochondrial translation and oxidative phosphorylation.TRMU is associated with several genetic disorders. Mutations in the TRMU gene can cause mitochondrial diseases, such as infantile hepatic failure syndrome. (PMID:23625533)

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