Anticorps Recombinant de lapin anti-TRNP1

• Phare
• Validé par KD/KO

TRNP1 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240331C6

N° de cat : 83410-3-RR

Synonymes

240331C6, C1orf225, TMF-regulated nuclear protein 1, TNRP, TRNP

---

Galerie de données de validation

• 

  WB analysis using 83410-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83410-3-RR (TRNP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 83410-3-RR

  WB result of TRNP1 antibody (83410-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRNP1 transfected HeLa cells.

• 

  FC experiment of HeLa using 83410-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TRNP1 Recombinant antibody (83410-3-RR, Clone:240331C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83410-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83410-3-RR against Human TRNP1 were performed. The affinity constant is 72.8 pM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules SH-SY5Y, tissu cardiaque de rat
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83410-3-RR cible TRNP1 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRNP1 Protéine recombinante Ag22504
• Nom complet: TMF1-regulated nuclear protein 1
• Masse moléculaire calculée: 227 aa, 23 kDa
• Poids moléculaire observé: 23-25 kDa
• Numéro d’acquisition GenBank: BC069216
• Symbole du gène: TRNP1
• Identification du gène (NCBI): 388610
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRNP1 (TMF-regulated nuclear protein 1), also called C1orf225, is a DNA-binding factor that regulates the expression of a subset of genes and plays a key role in tangential, radial, and lateral expansion of the brain neocortex (PMID: 27264089; 23622239; 36947129). It has been shown to exert potent roles in neural development affecting neural stem cell self-renewal and brain folding (PMID: 32627867). It may also control the proliferation rate of cells by regulating their progression through key cell-cycle transition points.

{{ptg:RelatedPrimaryAntibodies}}