Anticorps Polyclonal de lapin anti-TRPA1

TRPA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(31)

N° de cat : 19124-1-AP

Synonymes

ANKTM1, p120, Wasabi receptor

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Galerie de données de validation

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  WB analysis using 19124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 19124-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19124-1-AP (TRPA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat dorsal root ganglion using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19124-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19124-1-AP (TRPA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu de côlon de porc
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 24 publications below
• IHC: See 6 publications below
• IF: See 10 publications below

Informations sur le produit

19124-1-AP cible TRPA1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: transient receptor potential cation channel, subfamily A, member 1
• Masse moléculaire calculée: 140 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_007332
• Symbole du gène: TRPA1
• Identification du gène (NCBI): 8989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds.

Publications

Species	Application	Title
mouse	WB,IF	Metabolism; IFT80 and TRPA1 cooperatively regulate bone formation by calcium signaling in response to mechanical stimuli; Authors - Ting Wang
rat	WB	Phytomedicine; Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis; Authors - Wenjia Chen
mouse	IHC	J Control Release; A dual receptors-targeting and size-switchable "cluster bomb" co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer.; Authors - Yashi Wang
		Front Pharmacol; The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae; Authors - Nan-Nan Liu
rat,human	WB,IF	J Ethnopharmacol; Ligustilide covalently binds to Cys703 in the pre-S1 helix of TRPA1, blocking the opening of channel and relieving pain in rats with acute soft tissue injury; Authors - Kaixue Zhang
mouse	WB,IF	Mol Nutr Food Res; Menthol from Mentha piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.; Authors - Norihiro Suzuki

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