Anticorps Polyclonal de lapin anti-TRPM4

TRPM4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21985-1-AP

Synonymes

hTRPM4, LTrpC 4, LTrpC4, Melastatin 4, TRPM4, TRPM4B

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Galerie de données de validation

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  WB analysis using 21985-1-AP

  HEK-293 cells( total protein heated at 100℃, membrane protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21985-1-AP

  HEK-293 cells lysates (total protein heated at 100℃, total protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21985-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21985-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: 37°C incubated HEK-293 cells,
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

21985-1-AP cible TRPM4 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPM4 Protéine recombinante Ag17132
• Nom complet: transient receptor potential cation channel, subfamily M, member 4
• Masse moléculaire calculée: 1214 aa, 134 kDa
• Poids moléculaire observé: 130~150 kDa
• Numéro d’acquisition GenBank: BC132727
• Symbole du gène: TRPM4
• Identification du gène (NCBI): 54795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPM4 is a Calcium-activated nonselective cation channel. It is activated by increase in intracellular Ca2+, mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. TRPM4 participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. It's also involved in myogenic constriction of cerebral arteries.

Publications

Species	Application	Title
human	IF	Comput Struct Biotechnol J; Identification of a 24-gene panel and a novel marker of PODXL2 essential for the pathological diagnosis of early prostate cancer; Authors - Xiaoshi Ma
human	WB	Tissue Cell; Transient receptor potential channel TRPM4 favors oxidized low-density lipoprotein-induced coronary endothelial cell dysfunction via a mechanism involving ferroptosis; Authors - Fengxiang Ye ; KD Validated

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