Anticorps Polyclonal de lapin anti-TRPM4

TRPM4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21985-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 21985-1-AP (same clone as 21985-1-PBS)

  HEK-293 cells( total protein heated at 100℃, membrane protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

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  WB analysis using 21985-1-AP (same clone as 21985-1-PBS)

  HEK-293 cells lysates (total protein heated at 100℃, total protein incubated at 37℃) were subjected to SDS PAGE followed by western blot with 21985-1-AP (TRPM4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21985-1-AP (same clone as 21985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21985-1-AP (same clone as 21985-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21985-1-AP (TRPM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21985-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21985-1-PBS cible TRPM4 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPM4 Protéine recombinante Ag17132
• Nom complet: transient receptor potential cation channel, subfamily M, member 4
• Masse moléculaire calculée: 1214 aa, 134 kDa
• Poids moléculaire observé: 130~150 kDa
• Numéro d’acquisition GenBank: BC132727
• Symbole du gène: TRPM4
• Identification du gène (NCBI): 54795
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRPM4 is a Calcium-activated nonselective cation channel. It is activated by increase in intracellular Ca2+, mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. TRPM4 participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. It's also involved in myogenic constriction of cerebral arteries.

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