Anticorps Polyclonal de lapin anti-TRPM5

• Phare
• Validé par KD/KO

TRPM5 Polyclonal Antibody for WB, IHC, IF, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 18027-1-AP

Synonymes

LTrpC 5, LTrpC5, LTrpC-5, MLSN1- and TRP-related gene 1 protein, MTR1

---

Galerie de données de validation

• 

  WB analysis of mouse liver using 18027-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18027-1-AP (TRPM5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 18027-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18027-1-AP (TRPM5 antibody) at dilution of 1:200 (under 10x lens)..

• 

  IHC staining of human small intestine using 18027-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 18027-1-AP (TRPM5 antibody) at dilution of 1:200 (under 40x lens)..

• 

  IF Staining of mouse olfactory epithelium tissue using 18027-1-AP

  Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of TRPM5 antibody (18027-1-AP, 1:200). Microvillar cell staining in the apical layer. By Dr. Brian Lin (Schwob Lab).

• 

  FC experiment of LNCaP using 18027-1-AP

  1x10^6 LNCaP cells were intracellularly stained with 0.4 ug TRPM5 Polyclonal antibody (18027-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris,
• Résultats positifs en IHC: tissu d'intestin grêle humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'épithélium olfactif de souris
• Résultats positifs en FC (Intra): cellules LNCaP,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

18027-1-AP cible TRPM5 dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPM5 Protéine recombinante Ag12593
• Nom complet: transient receptor potential cation channel, subfamily M, member 5
• Masse moléculaire calculée: 98 kDa, 131 kDa
• Poids moléculaire observé: 98 kDa
• Numéro d’acquisition GenBank: BC093787
• Symbole du gène: TRPM5
• Identification du gène (NCBI): 29850
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transient receptor potential (TRP) proteins are a diverse family of proteins with structural features typical of ion channels (PMID: 14634208). TRPM5 is a member of the TRPM (melastatin-like) subfamily which are Ca(2+)-permeable cation channels localized predominantly to the plasma membrane (PMID: 11864597). TRPM5 plays a central role in taste transduction (PMID: 17610722). TRPM5 is implicated in enhancing TRPA1 expression and may be involved in regulating insulin secretion (PMID: 21932052). Alternative splicing results in transcript variants encoding distinct isoforms with calculated molecular weights of 98 kDa or 131 kDa. It has been reported that TRPM5 is N-linked glycosylated at a unique site and TRPM5 glycosylation seems not to be involved in channel trafficking, but mainly in its functional regulation (PMID: 24605085).

Publications

Species	Application	Title
mouse	WB	Immunity; Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.; Authors - Zhen Xiong
human		Food Funct; Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2.; Authors - Ting-Ting Huang ; KD Validated
rat	IF	Appetite; Chronic exposure to liquid sucrose and dry sucrose diet have differential effects on peripheral taste responses in female rats.; Authors - Lynnette Phillips McCluskey
human	IF	Heliyon; Involvement of pore helix in voltage-dependent inactivation of TRPM5 channel.; Authors - Kunitoshi Uchida
mouse,human	WB	Respir Res; Human airway tuft cells influence the mucociliary clearance through cholinergic signalling; Authors - Monika I Hollenhorst
mouse	WB,IF	Curr Med Sci; Insufficient TRPM5 Mediates Lipotoxicity-induced Pancreatic β-cell Dysfunction; Authors - Kai-Yuan Wang

{{ptg:RelatedPrimaryAntibodies}}