Anticorps Polyclonal de lapin anti-TRPS1

• Phare
• Validé par KD/KO

TRPS1 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IF-P, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 21938-1-AP

Synonymes

GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein

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Galerie de données de validation

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  WB analysis using 21938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21938-1-AP

  WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.

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  WB analysis of HeLa using 21938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21938-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 21938-1-AP

  IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

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  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse skin using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 21938-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), (CL488-Phalloidin, green).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu pulmonaire de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IF-P: tissu cutané de souris,
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

21938-1-AP cible TRPS1 dans les applications de WB, IF/ICC, IF-P, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPS1 Protéine recombinante Ag16583
• Nom complet: trichorhinophalangeal syndrome I
• Masse moléculaire calculée: 1294 aa, 143 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC125020
• Symbole du gène: TRPS1
• Identification du gène (NCBI): 7227
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

Publications

Species	Application	Title
human	WB	Cell Death Discov; The feedback loop between miR-222-3p and ZEB1 harnesses metastasis in renal cell carcinoma; Authors - Fan Wang
human	WB,ChIP	Cell Biosci; Tie2-expressing monocytes/macrophages promote angiogenesis in chronically ischaemic brain tissue; Authors - Chuyang Tai
human		Biochim Biophys Acta Mol Basis Dis; UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation; Authors - Yushan Zhang
mouse	IF	Acta Biomater; Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension; Authors - Danlan Fu
human	IF	Clin Transl Oncol; Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer; Authors - Dapeng Zhao

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